All Photos(2)

R4528

Sigma-Aldrich

Anti-hnRNP-A1 antibody, Mouse monoclonal

clone 9H10, purified from hybridoma cell culture

Synonym(s):
Anti-heterogeneous nuclear ribonucleoprotein-A1
MDL number:

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9H10, monoclonal

form

buffered aqueous solution

mol wt

antigen ~34 kDa

species reactivity

human, hamster, canine, mouse, bovine, monkey, rat

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-1.0 μg/mL using total cell extract of 293T cells

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

General description

hnRNP-A1 (heterogeneous nuclear ribonucleoprotein) is a pre-mRNA/mRNA binding protein, that belongs to the hnRNP family. It is located in chromosome 12q13.1. It is located in the nucleoplasm but it travels between the nucleus and the cytoplasm. It has two RNP motif RNA-binding domains (RBDs) at the amino terminus and a glycine-rich domain at the carboxyl terminus.

Immunogen

purified human hnRNP-A1.

Application

Anti-hnRNP-A1 has been used in immunofluorescence, immunohistochemistry and western blotting.

Does not immunoprecipitate the hnRNP complex.

Biochem/physiol Actions

hnRNP-A1 (heterogeneous nuclear ribonucleoprotein) participates in several RNA metabolisms. It also participates in deep vein thrombosis patients with behçet′s disease. hnRNPA1 mutations results in amyotrophic lateral sclerosis and multisystem proteinopathy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Quotes and Ordering

Carolina Villarroya-Beltri et al.
Nature communications, 4, 2980-2980 (2013-12-21)
Exosomes are released by most cells to the extracellular environment and are involved in cell-to-cell communication. Exosomes contain specific repertoires of mRNAs, microRNAs (miRNAs) and other non-coding RNAs that can be functionally transferred to recipient cells. However, the mechanisms that
A nuclear localization domain in the hnRNP A1 protein
Siomi H and Dreyfuss G
The Journal of Cell Biology, 129(3), 551-560 (1995)
HnRNP A1 is Involved in Deep Vein Thrombosis Patients with Behcet's Disease
Chen P, et al.
EBioMedicine, 6, 215-221 (2016)
Novel somatic single nucleotide variants within the RNA binding protein hnRNP A1 in multiple sclerosis patients
Lee S and Levin M
F1000Research, 3:132 (2014)
hnRNPA1 autoregulates its own mRNA expression to remain non-cytotoxic
Suzuki H and Matsuoka M
Molecular and Cellular Biochemistry, 427(1-2), 123-131 (2017)

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