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R4407

Sigma-Aldrich

Anti-RER1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-RER1 retention in endoplasmic reticulum 1
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~25 kDa

species reactivity

rat, mouse, human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 1-2 μg/mL using mouse 3T3 and rat NRK cells
western blot: 3-6 μg/mL using whole extract of HEK-293T cells expressing human Rer1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... RER1(11079)
mouse ... Rer1(67830)
rat ... Rer1(98675)

General description

Retention in endoplasmic reticulum 1 (Rer1p) is found in Golgi-compartment. The protein contains four transmembrane domains (TDMs), with the amino and carboxy termini facing the cytosol and recognizes polar amino acids in TMDs of several proteins including sec12p and sec71p.

Specificity

Anti-Rer1 recognizes human, mouse, and rat Rer1.

Application

Anti-RER1 antibody produced in rabbit has been used in:
  • immunoblotting
  • immunohistochemistry
  • immunofluorescence

Anti-RER1 antibody produced in rabbit may be used in immunoblotting, immunofluorescence and immunohistochemical (IHC) techniques. It is used to determine the roles of RER1 in the retention/retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment.

Biochem/physiol Actions

Retention in endoplasmic reticulum 1 (Rer1) is essential for retrieval of endoplasmic reticulum (ER) membrane proteins from the early Golgi compartment. In addition, mammalian Rer1 is also involved in the ER retention/retrieval of unassembled γsecretase complex subunits. Rer1 interacts with immature nicastrin and unassembled Pen2 through critical residues found in their TMDs. Downregulation of protein expression enhances surface localization of Pen2, whereas Upregulated expression of Rer1 stabilizes unassembled Pen2. Thus, Rer1 regulates the assembly of the γ-secretase complex and therefore contributes to total cellular γ-secretase activity.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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