OGS589

PBR322 - PBR322 LOW COPY CLONING VECTOR

plasmid vector for molecular cloning

• Synonym(s): cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• UNSPSC Code: 12352200
• NACRES: NA.85

Properties

• form: buffered aqueous solution
• mol wt: size 4361 bp
• bacteria selection: ampicillin
• origin of replication: BR322 (15 copies)
• peptide cleavage: no cleavage
• reporter gene: none
• shipped in: ambient
• storage temp.: −20°C

Description

General description

This plasmid contains the BR322 (low copy number) origin of replication and provides approximately 20 plasmid copies per bacterial cell. It also contains the mammalian CMV promoter upstream of the MCS and Kan resistance for selection of transfected mammalian cells. Low copy number plasmids are useful for very large transgenes or for transgenes that can be toxic to bacterial cells.

Promoter Expression Level:

Application

BR322 Information: BR322 is a wild type plasmid isolated from E.coli. It contains a low copy origin that allows for plasmid maintenance at a frequency of approximately 10-100 plasmid copies per cell. Typically this number is closer to 30-40 copies per cell. Most common cloning plasmids contain a derivative of the origin of replication in this plasmid called pUC. The pUC origin was created by removing the Rep repressor protein that normally regulates plasmid copy number in E.coli and also making a single point mutation in the origin of replication itself. The point mutation is the most important difference between the origin of replication.This plasmid can be used as a general purpose low copy cloning vector.

Sequence

To view sequence information for this product, please visit the product page

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable