cell culture | mammalian: suitable
endotoxin and total aerobic microbial count, tested
Fe: ≤5 ppm
≤0.05 at 260 in H2O at 0.1 M
≤0.05 at 280 in H2O at 0.1 M
diagnostic assay manufacturing
DNase, RNase, NICKase, protease, none detected
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This protocol describes a method for chemical cross-linking of proteins using formaldehyde. With the exception of zero-length cross-linkers, formaldehyde has the shortest cross-linking span (~2-3 Å) of any cross-linking reagent, thus making it an ideal tool for detecting specific protein-protein interactions with great confidence.
cAMP measurements are obtained using an ELISA assay (Harlow and Lane 1988). Commercial radio-immunoassays, or ELISA kits, to assay cAMP can be purchased from various manufacturers.
To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.