H4034

HEPES

BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• EC Number: 230-907-9
• MDL number: MFCD00006158
• PubChem Substance ID: 24895616
• NACRES: NA.25

Properties

• grade: BioPerformance Certified
• Quality Level: 400
• assay: ≥99.5% (titration)
• form: crystalline powder
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin and total aerobic microbial count, tested
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• cation traces: Fe: ≤5 ppm
• absorption: ≤0.05 at 260 in H₂O at 0.1 M; ≤0.05 at 280 in H₂O at 0.1 M
• application(s): diagnostic assay manufacturing
• foreign activity: DNase, RNase, NICKase, protease, none detected
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.[12]

HEPES has been described as one of the best all-purpose buffers available for biological research.[35] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[36]

Application

HEPES has been used:

• To supplement Dulbecco′s modified Eagle′s medium to culture and maintain cell lines

[46][48]

As a component of platelet suspension buffer

[47]

To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue

[49]

As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay

[67]

For the adjustment and maintenance of pH of biological solutions

[67]

As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development

[65]

For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells

[15]

As a component of keratinocyte and fibroblast culture medium

[99]

Packaging

100, 500 g in poly bottle

1 kg in poly bottle

25 kg in poly drum

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves