MilliporeSigma
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H6147

Sigma-Aldrich

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

product line

BioXtra

Quality Level

assay

≥99.5% (titration)

form

powder

technique(s)

cell culture | embryo: suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5
7.5

solubility

water: 500 mg/mL, clear, colorless

absorption

≤0.05 at 290 in H2O at 33%

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[35] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[36] It is typically used in cell culture at concentration between 5mM to 30mM.

Application

HEPES has been used:
  • As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
[98]
  • As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
  • [99]
  • in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation
  • [00]

    Packaging

    100, 500 g in poly bottle

    Other Notes

    This product has been qualified for use in cell and mouse embryo culture.
    Easily compare specifications for HEPES products with the HEPES specification table.

    Physical properties

    Useful pH range: 6.8 - 8.2

    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 1

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

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    Certificate of Origin

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