≥99.5% (titration)

N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


≥99.5% (titration)


crystalline powder


cell culture | mammalian: suitable


5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8 - 8.2

pKa (25 °C)



water: 500 mg/mL, clear, colorless

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Diagnostic Assay Manufacturing

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General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.


HEPES has been used:
  • As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
  • As a component of Hanks′ solution used in the degradation testing of Iron
  • As a component of HEPES medium in the transfection of cell culture with plasmids
  • Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
  • As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies


5, 25, 50 kg in poly drum
25, 100, 250, 500 g in poly bottle
1 kg in poly bottle

Other Notes

Go to Redi-Dri Product Listing RDD002,
Easily compare specifications for HEPES products with the HEPES specification table,.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the purpose of using HEPES in cell culture?

    HEPES provides supplemental buffering for media in pH range of 7.2 to 7.6. It has no nutritional value to cells.

  4. What is the concentration of HEPES used in cell culture?

    HEPES is typically used in cell culture in the range of 10-25 mM.

  5. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Caveolae are highly immobile plasma membrane microdomains, which are not involved in constitutive endocytic trafficking.
Thomsen P, et al.
Molecular Biology of the Cell, 13(1), 238-250 (2002)
Culturing INS-1 cells on CDPGYIGSR-, RGD-and fibronectin surfaces improves insulin secretion and cell proliferation.
Kuehn C, et al.
Acta Biomaterialia, 8(2), 619-626 (2012)
Structural, metabolic and developmental evaluation of ovulated rabbit oocytes before and after cryopreservation by vitrification and slow freezing.
Salvetti P, et al.
Theriogenology, 74(5), 847-855 (2010)
Electroformed pure iron as a new biomaterial for degradable stents: In vitro degradation and preliminary cell viability studies.
Moravej M, et al.
Acta Biomaterialia, 6(5), 1843-1851 (2010)
High-resolution in situ hybridization to whole-mount zebrafish embryos.
Thisse C and Thisse B
Nature Protocols, 3(1), 59-69 (2008)
rganoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
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