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H3375

Sigma-Aldrich

HEPES

≥99.5% (titration)

Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

assay

≥99.5% (titration)

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 500 mg/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[35] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[36]

Application

HEPES has been used:
  • As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
[39]
  • As a component of Hanks′ solution used in the degradation testing of Iron
  • [38]
  • As a component of HEPES medium in the transfection of cell culture with plasmids
  • [39]
  • Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
  • [40]
  • As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies
  • [41]

    Packaging

    25, 100, 250, 500 g in poly bottle
    1 kg in poly bottle
    5, 25, 50 kg in poly drum

    Other Notes

    Go to Redi-Dri Product Listing RDD002
    Easily compare specifications for HEPES products with the HEPES specification table.

    Legal Information

    Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

    Storage Class Code

    13 - Non Combustible Solids

    WGK

    WGK 1

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

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    Certificate of Origin

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