54457

HEPES

BioUltra, for molecular biology, ≥99.5% (T)

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• EC Number: 230-907-9
• MDL number: MFCD00006158
• PubChem Substance ID: 57651473
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• product line: BioUltra
• Assay: ≥99.5% (T)
• form: powder
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• ign. residue: ≤0.1% (as SO₄)
• loss: ≤0.5% loss on drying, 110 °C
• pH: 5.0-6.5 (25 °C, 1 M in H₂O)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubility: H₂O: 1 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 1 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.045; λ: 280 nm A_max: 0.035
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used as a component of:

• DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
• RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
• Recapture solution to re-capture RNA exosomes from the glycerol gradient
• ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
• 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

3′-Terminal labeling of RNA with T₄ RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves