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83264

Sigma-Aldrich

HEPES buffer solution

1 M in H2O

Synonym(s):
HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
CAS Number:
MDL number:
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25

form

liquid

Quality Level

concentration

1 M in H2O
238 g/L HEPES in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.5±0.5

useful pH range

6.8-8.2

density

1.07 g/mL at 20 °C

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[35] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[36]

Application

HEPES has been used:
  • As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
[15]
  • To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
  • [61][62]
  • As a component of dissection buffer for culturing rat cortical neurons
  • [63]
  • Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
  • [64]
  • As a component of Danieu′s aquarium water used in agarose gel preparation
  • [65]

    Storage Class Code

    12 - Non Combustible Liquids

    WGK

    WGK 1

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

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    Certificate of Origin

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