46043

Ethidium homodimer

suitable for fluorescence, ~90% (HPCE)

• Synonym(s): EtDi, EthD-1
• Empirical Formula (Hill Notation): C₄₆H₅₀Cl₄N₈
• CAS Number: 61926-22-5
• Molecular Weight: 856.75
• EC Number: 263-325-9
• MDL number: MFCD00210948
• UNSPSC Code: 12161505
• PubChem Substance ID: 329757072
• NACRES: NA.32

Properties

• assay: ~90% (HPCE)
• Quality Level: 100
• form: solid
• mp: ≥250 °C (lit.)
• solubility: DMF: soluble; DMSO: soluble; H₂O: soluble
• fluorescence: λ_ex 493 nm in H₂O; λ_ex 528 nm; λ_em 617 nm in TE buffer; DNA
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28
• InChI: 1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
• InChI key: ZKAJUKABUUJRLB-UHFFFAOYSA-N

Description

Application

Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.

Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Reagent for the fluorimetric detection of nucleic acids

Safety Information

• pictograms: GHS02,GHS08,GHS05
• signalword: Danger
• hcodes: H261,H314,H351
• pcodes: P231 + P232 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Carc. 2 - Eye Dam. 1 - Skin Corr. 1B - Water-react 3
• Storage Class: 4.3 - Hazardous materials which set free flammable gases upon contact with water
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves