solution (1.0 mg/ml in water)
water: 1 mg/mL
5400-6400 at 491-495 nm in phosphate buffer
Diagnostic Assay Manufacturing
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Propidium Iodide does not generally enter living cells, However it may be taken up by axonal terminals and retrogradely transported to neuronal cell bodies. Reference: Aschoff, A. and H. Hollander, Fluorescent compounds as retrograde tracers compared with horseradish peroxidase (HRP). I. A parametric study in the central visual system of the albino rat. J. Neurosci. Methods, 6(3), 179-197 (1982).
Immediately before flow cytometric analysis, prepare a 0.5 mg/mL solution of propidium iodide by combining 50μL of 2X PBS with 50 μL of P4864. Add this solution to 1 mL of a cell suspension containing 10,000,000 cells. (Note: Cells exhibiting fluorescence above 630 nm should be excluded from further analysis.)
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