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C1359

Sigma-Aldrich

Calcein AM solution

4 mM in DMSO, ≥90% (HPLC), solution

Empirical Formula (Hill Notation):
C46H46N2O23
CAS Number:
Molecular Weight:
994.86
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥90% (HPLC)

form

solution

concentration

4 mM in DMSO

λmax

491-497 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45

InChI

1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3

InChI key

XKFSBWQWNMZWFA-UHFFFAOYSA-N

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Application

In cells, calcein AM solution has been used in calcein-AM dye transfer assay. It has been used for the staining of cells to study quantification of cell-migration. It has been used to study cytotoxic effects of drugs in cells.

Packaging

100 μL in glass bottle

Biochem/physiol Actions

Fluorescent cell permeable derivative of calcein.
Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.

Physical form

Solution in 0.4% Calcein AM and 99.6% DMSO.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

185.0 °F - closed cup

Flash Point(C)

85 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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