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Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

Fluorescein-bis(methyliminodiacetic acid), Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorexon
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
PubChem Substance ID:

Quality Level


mixed isomers




titration: suitable


NaOH: 50 mg/mL


λex 470 nm; λem 509 nm (pH 7.4)


diagnostic assay manufacturing

storage temp.

room temp

SMILES string


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Calcein has been used:
  • in whole cell staining
  • in histomorphometry and micro-computed tomography
  • to label active bone-forming surfaces


5, 10, 25 g in glass bottle

Biochem/physiol Actions

Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What are the excitation and emission wavelengths of Calcein, Product C0875?

    Conn's Biological Stains, 10th Edition states excitation is at 494 nm in aqueous solutions at pH 9; the emission maximum is 517 nm. Depending upon the pH of the solution, slightly different values have been reported in the literature.

  4. Is there a reference for using Calcein, Product C0875, to study bone growth?

    Clement-Lacroix, P., et al. Osteoblasts are a new target for prolactin: analysis of bone formation in prolactin receptor knockout mice. Endocrinology, 140, 96-105 (1999).

  5. What is the extinction coefficient of Calcein, Product C0875?

    For samples in 1 M sodium hydroxide: E1% = 1040 at 500-504 nm; E1% = 466 at 242-245 nm.

  6. What is the solubility of Calcein, Product C0875?

    Calcein is soluble at a concentration of 50 mg/mL in 1M sodium hydroxide, yielding clear orange to brown solution.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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