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C0875

Sigma-Aldrich

Calcein

Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

Synonym(s):
Fluorescein-bis(methyliminodiacetic acid), Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorexon
Empirical Formula (Hill Notation):
C30H26N2O13
CAS Number:
Molecular Weight:
622.53
Beilstein:
8181068
PubChem Substance ID:
NACRES:
NA.47

Quality Level

description

mixed isomers

form

powder

technique(s)

titration: suitable

solubility

NaOH: 50 mg/mL

fluorescence

λex 470 nm; λem 509 nm (pH 7.4)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O

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Application

Calcein has been used:
  • in whole cell staining
  • in histomorphometry and micro-computed tomography
  • to label active bone-forming surfaces

Packaging

5, 10, 25 g in glass bottle

Biochem/physiol Actions

Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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