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BioReagent, suitable for fluorescence, ≥95.0% (HPLC)

Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester, Calcein acetoxymethyl ester
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
MDL number:
PubChem Substance ID:

Quality Level

product line



≥95.0% (HPLC)




λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+


suitable for fluorescence

storage temp.


SMILES string




InChI key


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General description

Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.


Calcein-AM has been used in flow cytometry.
Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research.

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the solubility of Calcein-AM, Product 17783?

    Sigma tests the solubility of Product No. 17783 at a concentration of 10 mg/mL in dimethyl sulfoxide (DMSO).

  6.  What is the appearance of Calcein-AM, Product No. 17783?

    Product No. 17783 can be a film, oil, paste or solid.

  7. What are the spectral properties of Calcein-AM, Product 17783?

    The emission maximum for Calcein-AM is 516 nm, with excitation at 496 nm in 0.1M Tris pH 8.0 (following hydrolysis by esterase enzyme, bound to Ca(2+)).

  8. How is Calcein-AM, Product 17783, typically used?

    Calcein-AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability. In live cells the non-fluorescent calcein-AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells.

  9. Do the Scienceware® brand polypropylene products contain any leachable material?  

    Although Bel-Art does not specifically test their Scienceware® brand polypropylene products, they have no evidence of any problems with leachable material in the polypropylene.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

T M Allen et al.
Biochimica et biophysica acta, 597(2), 418-426 (1980-04-10)
Efflux of contents from small unilamellar vesicles of various compositions, conaining a highly quenched fluorescent compound (calcein, 175 mM) was determined as a function of temperature in the presence and absence of human serum. Efflux of calcein from the liposomes
Y S Tsao et al.
Biochemistry, 24(5), 1092-1098 (1985-02-26)
Sendai virus induced liposome leakage has been studied by using liposomes containing a self-quenching fluorescent dye, calcein. The liposomes used in this study were prepared by a freeze and thaw method and were composed of phosphatidylcholine, phosphatidylserine, and phosphatidylethanolamine (1:2.60:1.48
Xiao-Cong Huang et al.
Biochemical pharmacology, 85(9), 1257-1268 (2013-02-19)
High intrinsic or acquired expression of membrane spanning, adenosine triphosphate binding cassette (ABC) transporter proteins, such as P-glycoprotein (P-gp), in cancers represents a major impediment to chemotherapy, with accelerated drug efflux leading to multi-drug resistance (MDR). Although ABC transporter inhibitors
Bao-Ling Du et al.
Journal of biomedical materials research. Part A, 103(4), 1533-1545 (2014-07-22)
Biological materials combined with genetically-modified neural stem cells (NSCs) are candidate therapy targeting spinal cord injury (SCI). Based on our previous studies, here we performed gelatin sponge (GS) scaffold seeded with neurotrophin-3 (NT-3) and its receptor TrkC gene modifying NSCs
Monika Enqvist et al.
Journal of immunology (Baltimore, Md. : 1950), 194(9), 4518-4527 (2015-04-01)
The functional capacity of NK cells is dynamically tuned by integrated signals from inhibitory and activating cell surface receptors in a process termed NK cell education. However, the understanding of the cellular and molecular mechanisms behind this functional tuning is


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