I0634

Ionomycin calcium salt from Streptomyces conglobatus

powder, ≥98% (HPLC)

• Synonym(s): SQ23377 calcium
• Empirical Formula (Hill Notation): C₄₁H₇₀CaO₉
• CAS Number: 56092-82-1
• Molecular Weight: 747.07
• Beilstein: 1416163
• MDL number: MFCD00083634
• PubChem Substance ID: 329815321
• NACRES: NA.77

Properties

• Quality Level: 300
• assay: ≥98% (HPLC)
• form: powder
• storage condition: desiccated; protect from light
• color: white to off-white
• solubility: DMSO: 10 mg/mL; ethanol: soluble
• antibiotic activity spectrum: Gram-positive bacteria
• Mode of action: cell membrane | interferes
• storage temp.: 2-8°C
• SMILES string: C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O
• InChI: 1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1
• InChI key: WKRWUYKLUMMAKG-WYGBAUISSA-L

Description

Application

Ionomycin calcium salt from Streptomyces conglobatus has been used as a media supplement to culture:

• peripheral blood mononuclear cells (PBMCs) for immunologic assessment
• oocytes for parthenogenetic activation
• T cell subsets for the detection of intracellular cytokines, carboxyfluorescein diacetate succinimidyl ester (CFSE) proliferation assay and antigen-specific suppressive function assay

Packaging

1, 5 mg in glass bottle

Biochem/physiol Actions

Ca²⁺ ionophore that is more effective than A23187 as a mobile ion carrier for Ca²⁺; non-fluorescent; used to study Ca²⁺ transport across biological membranes; induces apoptotic degeneration of embryonic cortical neurons.

Ionomycin is an ionophore, widely used for validation and calibration of calcium analysis.

Preparation Note

Store ionomycin in powder form desiccated and protected from light at 2-8 °C. Under these conditions the product is stable for 3 years. Store stock solutions in ethanol or DMSO at -20 °C, protected from light. Under these conditions the stock solutions are stable for several months. For short term use (up to 6 weeks) the stock solutions maybe stored at 2-8 °C protected from light.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302
• Precautionary Statements: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves