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Key Documents

AB763P

Sigma-Aldrich

Anti-Collagen Type V Antibody

Chemicon®, from rabbit

Synonym(s):

Anti-EDSC, Anti-FMDMF

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
radioimmunoassay: suitable

NCBI accession no.

NM_000093.3

UniProt accession no.

P20908

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... COL5A1(1289)

General description

Collagen Type V extracted and purified from human placenta. Antibody shows less than 0.1% reactivity with human Collagen Types I, III, and IV. AB763P also shows 0.1% reactivity with human Fibronectin and mouse Laminin by radioimmunoassay.

Specificity

Human collagen type V: 100%

Human collagen type I, II, & III: <0.5%

Human collagen type IV: 20%

Human fibronectin, albumin, immunoglobulins: <0.5%.

Application

Anti-Collagen Type V Antibody detects level of Collagen Type V & has been published & validated for use in ELISA, IF, RIA.
Immunohistochemistry: 1:40 dilution for immunofluorescent staining of frozen human skin, liver and placenta tissues.

Radio Immunoassay

ELISA

Optimal working dilution must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Physical form

Format: Purified
The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µl/ml of antibiotics and antimycotics.

Storage and Stability

Maintain frozen at -20°C for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Colorectal cancer desmoplastic reaction up-regulates collagen synthesis and restricts cancer cell invasion.
Vivien J Coulson-Thomas,Yvette M Coulson-Thomas,Tarsis F Gesteira,Claudia A A de Paula et al.
Cell and Tissue Research null
Vivien Jane Coulson-Thomas
Bio-protocol, 7(4), e2131-e2131 (2017-02-20)
We have developed a 2D heterotypic co-culture technique between fibroblasts and cancer cells that enables the study of the stromal reaction. For such, stromal cells are seeded and cultured immediately around a tumour cell line, and the cells establish cell-cell

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