Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

L0418

Sigma-Aldrich

LB Agar Carbenicillin - 100, Plates

pre-poured agar plates with 100 μg/ml carbenicillin

Synonym(s):

carbenicillin agar plates

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41121800

grade

for molecular biology

Quality Level

sterility

sterile; autoclaved

form

plates (10 cm)
plates (10cm, 20-25ml each)

availability

available only in USA

capacity

20-25 mL

storage temp.

2-8°C

suitability

selective for Escherichia coli
selective for coliforms

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

LB Agar Carbenicillin - 100, Plates may be used to culture transformed bacteria with an antibiotic-resistant plasmid using a microwave oven.
Suitable for selective cultivation of E. coli strains that contain plasmids conferring carbenicillin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins.

Features and Benefits

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

Components

15 g/L Agar
10 g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
100 μg/ml carbenicillin

Other Notes

The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Sterile preparation of antibiotic-selective LB agar plates using a microwave oven
Iacoviello Michael P and Rubin SA
Biotechniques, 30(5), 963-965 (2001)

Articles

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service