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T6884
Thrombin from human plasma
lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3)
Synonim(y):
Factor IIa
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About This Item
Polecane produkty
pochodzenie biologiczne
human plasma
Poziom jakości
Formularz
lyophilized powder
aktywność właściwa
≥2,000 NIH units/mg protein (E1%/280, 18.3)
masa cząsteczkowa
37.4 kDa
zanieczyszczenia
HIV and HBsAg, source material tested negative
numer dostępu UniProt
temp. przechowywania
−20°C
informacje o genach
human ... F2(2147)
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Opis ogólny
Thrombin is produced from the proteolytic cleavage of inactive prothrombin in the liver. It is a glycoprotein with four structural domains: the 10 γ-carboxylated glutamic acid containing Gal domain, two kringle domains, and the trypsin-like serine protease domain. The prothrombin gene is mapped to human chromosome 11p11.2.
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
Zastosowanie
Thrombin from human plasma has been used:
- in the preparation of fibrin gels for assessing human bone marrow stromal cells (hBMSC) morphology,
- in the activation of platelets,
- to evaluate the integrity of endothelial cell (EC) monolayers
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags.
Działania biochem./fizjol.
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
The main function of thrombin is the cleavage of fibrinogen to fibrin, to assist stable clot formation. A wide range of mutations in the prothrombin gene contributes to its deficiency resulting in coagulation disorders like dysprothrombinemia and hypoprothrombinemia. High levels of thrombin elicit neurotoxicity in dopaminergic neurons and contributes to the progression of Parkinson′s disease. Altered thrombin levels modulates the coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin. Thrombin accumulation in neurofibrillary tangles of the brain may contribute to the aggregation of tau protein and pathophysiology of Alzheimer disease.
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to investigate the protein C pathway in intestinal barrier function.
Definicja jednostki
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
Postać fizyczna
Lyophilized from saline sodium citrate buffer, pH 6.5
Komentarz do analizy
The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Inne uwagi
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Oświadczenie o zrzeczeniu się odpowiedzialności
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Inhibitor
Numer produktu
Opis
Cennik
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organy docelowe
Respiratory system
Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Produkty
Czynnik trombinowy IIa jest endolityczną proteazą serynową, która selektywnie rozszczepia wiązania Arg--Gly fibrynogenu, tworząc fibrynę i uwalniając fibrynopeptydy A i B.
Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
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