T6634

Thrombin from bovine plasma

lyophilized powder, 600-2,000 NIH units/mg protein (biuret)

• Synonim(y): Factor IIa
• Numer CAS: 9002-04-4
• Numer EC enzymu: 3.4.21.5 (BRENDA, IUBMB)
• Numer WE: 232-648-7
• Numer MDL: MFCD00166053
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• Postać: lyophilized powder
• Poziom jakości: 300
• aktywność właściwa: 600-2,000 NIH units/mg protein (biuret)
• masa cząsteczkowa: heavy chain ~33 kDa; light chain ~5 kDa
• numer dostępu UniProt: P00735
• Zastosowanie: diagnostic assay manufacturing
• temp. przechowywania: −20°C
• informacje o genach: cow ... F2(280685)

Opis

Opis ogólny

Thrombin is a sodium-activated type II enzyme. It contains two anion binding exosites, ABE-I and ABE-II. The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Bovine a-thrombin consists of a light chain (A chain) and a heavy chain (B chain). These two chains are joined by one disulfide bond. The B chain of a-thrombin includes a carbohydrate portion.

Zastosowanie

Thrombin from bovine plasma has been used to study its effect on the perinatal rat subventricular zone cells and oligodendrocyte precursor cell proliferation, differentiation, and migration in culture. It has also been used in fibrin degradation assay to measure nattokinase activity.

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess global haemostasis and point of care testing.

Działania biochem./fizjol.

Thrombin is a proteolytic enzyme critical in the blood clotting process and activates clotting factors V, VIII, XI, and XII. Thrombin promotes platelet aggregation. Therefore, thrombin is the final coagulation protease in hemostasis, promoting both procoagulant and anticoagulant effects. It is used to treat bleeding from capillaries and small venules.

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Definicja jednostki

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

Postać fizyczna

Lyophilized from saline sodium citrate buffer, pH 6.5

Komentarz do analizy

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Inne uwagi

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H315,H319,H334,H335
• Zwroty wskazujące środki ostrożności: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable