A6191

Antipain dihydrochloride

lyophilized powder

• Synonim(y): N-(Nα-Carbonyl-Arg-Val-Arg-al)-Phe
• Wzór empiryczny (zapis Hilla): C₂₇H₄₄N₁₀O₆ · 2 HCl
• Numer CAS: 37682-72-7
• Masa cząsteczkowa: 677.62
• Numer MDL: MFCD00135957
• Kod UNSPSC: 12352202
• Identyfikator substancji w PubChem: 24891067
• NACRES: NA.77

Właściwości

• Nazwa produktu: Antipain dihydrochloride from microbial source, protease inhibitor
• pochodzenie biologiczne: Streptomyces sp.
• Poziom jakości: 300
• Formularz: lyophilized powder
• siła działania: 1.4 μM K_i
• rozpuszczalność: H₂O: 50 mg/mL; 1-butanol: soluble; 1-propanol: soluble; DMSO: soluble; ethanol: soluble; methanol: soluble
• Tryb działania: enzyme | inhibits
• temp. przechowywania: −20°C
• ciąg SMILES: Cl[H].Cl[H].[H]C(=O)C(CCCNC(N)=N)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(N)=N)NC(=O)NC(Cc1ccccc1)C(O)=O)C(C)C
• InChI: 1S/C27H44N10O6.2ClH/c1-16(2)21(23(40)34-18(15-38)10-6-12-32-25(28)29)37-22(39)19(11-7-13-33-26(30)31)35-27(43)36-20(24(41)42)14-17-8-4-3-5-9-17;;/h3-5,8-9,15-16,18-21H,6-7,10-14H2,1-2H3,(H,34,40)(H,37,39)(H,41,42)(H4,28,29,32)(H4,30,31,33)(H2,35,36,43);2*1H/t18?,19-,20?,21-;;/m0../s1
• Klucz InChI: YAHXZYICKJUJEO-BXLPLHKWSA-N

Opis

Opis ogólny

Chemical structure: peptide

Zastosowanie

Antipain dihydrochloride from microbial source has been used in the preparation of adipocyte proteins for western blotting and in protease inhibitor cocktail for isolating nuclear extracts for gelshift analysis.

Concentrations for 50% inhibition (μg/ml):
papain, 0.16
trypsin, 0.26
cathepsin A, 1.19
cathepsin B, 0.59
cathepsin D, 125
plasmin, >93
chymotrypsin and pepsin, >250
It also has been reported to inhibit calpain I, (porcine) with K_i = 1.4 μM

Działania biochem./fizjol.

Antipain dihydrochloride also inhibits the action of papain and cathespsin B.

Non-selective serine and cysteine protease inhibitor.

Reversible inhibitor of serine/cysteine proteases and some trypsin-like serine proteases. Its action resembles leupeptin; however, its plasmin inhibition is less and its cathepsin A inhibition is more than that observed with leupeptin. Chronic administration of antipain can reduce the frequency of chemically induced transformation in BALB/c-/3T3 cells.

Uwaga dotycząca przygotowania

Solubility testing at 50 mg/ml in water yields a clear to slightly hazy yellow solution. It is reportedly soluble in methanol, water, and DMSO; less soluble in ethanol, butanol, and propanol; insoluble in benzene, hexane, and chloroform.⁸ A stock solution in water or buffer is stable for about a month at -20 °C.

Dilute solutions should be stored on ice and kept for only a day because of the terminal aldehyde, which is subject to oxidation and racemization.

Stock solutions in water or buffer stable for 1 week at 4 °C, 1 month at −20 °C.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)