RECOMT
Thrombin CleanCleave™ Kit
Synonim(y):
Zestaw do rozszczepiania trombiny
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About This Item
Kod UNSPSC:
12352200
NACRES:
NA.56
Polecane produkty
Formularz
suspension
masa cząsteczkowa
37 kDa
metody
protein purification: suitable
Warunki transportu
wet ice
temp. przechowywania
2-8°C
Opis ogólny
The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins
Zastosowanie
For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.
Cechy i korzyści
- Fast, efficient cleavage in as little as 2 hours
- Thrombin is covalently bound to agarose for easy removal.
- The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
- Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
- Thrombin-agarose is reusable.
Ilość
200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.
Informacje prawne
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Tylko elementy zestawu
Numer produktu
Opis
- Thrombin Cleavage Buffer, 10× 10 mL
produkt powiązany
Numer produktu
Opis
Cennik
Kod klasy składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Protokoły
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