T1503

Trizma^® base

≥99.9% (titration), crystalline, primary standard, aminopeptidase substrate

• Synonim(y): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Wzór liniowy: NH₂C(CH₂OH)₃
• Numer CAS: 77-86-1
• Masa cząsteczkowa: 121.14
• Beilstein: 741883
• Numer WE: 201-064-4
• Numer MDL: MFCD00004679
• Kod UNSPSC: 12352104
• Identyfikator substancji w PubChem: 24899973
• NACRES: NA.25

Właściwości

• product name: Trizma^® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline
• Poziom jakości: 300
• opis: aminopeptidase substrate
• Próba: ≥99.9% (titration)
• Postać: crystalline
• warunki przechowywania: dry at room temperature
• metody: ELISA: suitable; protein extraction: suitable
• kolor: white
• pH: 10.5-12
• przydatny zakres pH: 7-9
• pKa (25°C): 8.1
• tw: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• rozpuszczalność: methanol: soluble 26 mg/mL at 25 °C; ethylene glycol: soluble 79.1 mg/mL at 25 °C; water: soluble (678 g/l at 20 °C)
• absorpcja: ≤0.05 at 290 nm at 40%
• przydatność: suitable for Western blot; suitable for electrophoresis
• Zastosowanie: cell analysis; diagnostic assay manufacturing; life science and biopharma
• ciąg SMILES: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• Klucz InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N

Opis

Opis ogólny

Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma^® base, serves a pivotal role in diverse research applications as a biological buffer. Its optimal pKa of 8.1 makes it a preferred choice for formulating buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), ensuring the maintenance of pH within the physiological range (pH 7 - 9), applicable to a wide spectrum of living organisms. Despite its utility, researchers need to exercise caution in protein studies, as Tris has the potential to interfere with the activity of specific enzymes.

Tris base may find application as basimetric standard, independently as a buffer and as a crucial component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, TBE buffer, among others. Its attributes include purity, essential stability, and a relative non-hygroscopic nature, making it a dependable choice in laboratory settings. In these environments, Tris base is indispensable for preparing buffers compatible with biological fluids and serves as a standard pH solution. It facilitates various laboratory procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. The versatility of Tris base extends to cell biology, biochemistry, and protein research contributing significantly to studies involving cell membrane permeability and buffer preparation.

Zastosowanie

Trizma^® base has been used:

• as a component of H buffer (cell dissociation buffer)
• for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique
• as an assay buffer for reconstitution of extracted and dried protein samples
• to prepare Tris-HCl buffer that is used to stabilize proteins
• as a buffer to extract carotenoid from tubers
• as a component of sample buffer during protein extraction prior to western blotting
• as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
• to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
• as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate

Cechy i korzyści

• Efficient buffering within the pH range of 7 - 9 with a pKa of 8.1 (25 °C)
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Can be used in Cell Biology, and Biochemical research

Inne uwagi

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

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Informacje prawne

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves