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Merck

L0777

Sigma-Aldrich

Lipase from Aspergillus oryzae

solution, ≥100,000 U/g, white, beige

Synonim(y):

AOL, Lipolase 100L

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About This Item

Numer CAS:
Numer EC enzymu:
3.1.1.3.
Numer WE:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.54

pochodzenie biologiczne

Aspergillus sp. (Aspergillus oryzae)

Poziom jakości

Formularz

solution

aktywność właściwa

≥100,000 U/g

warunki przechowywania

(Tightly closed. Dry)

metody

cell based assay: suitable

kolor

beige
white

numer dostępu UniProt

temp. przechowywania

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

Klucz InChI

QWZUIMCIEOCSJF-CHHCPSLASA-N

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Opis ogólny

Research area: Cell Signaling

Lipase is derived from Aspergillus oryzae by ammonium sulfate precipitation and chromatography.Lipases or triacylglycerol acyl hydrolases are a group of hydrolase enzymes that are usually found in humans and animals with monogastric stomachs. Lipase enzymes are generally formed in the pancreas and stomach where they aid in fat and lipids digestion.(2)

Zastosowanie

Lipase from Aspergillus oryzae has been used:
  • as a control enzyme in activity assays
  • as a component in lipolase stock solution for the hydrolysis experiments
  • to incubate scaffolds for lipase-accelerated degradation experiments
  • as the lipolytic enzyme standard to detect lipolytic enzymatic activity via chromogenic agar plates and zymography

Działania biochem./fizjol.

Lipase is widely used in several industries including food and pharmaceuticals. It mediates the hydrolysis of fats and oil. Lipase is utilized for peptide synthesis and in the detergent industry. It is active in the range of pH 2-5 and temperature between 30-50°C. Metal ions such as Fe2+, Fe3+, and Cu2+ prevent the action of lipase. A less polar organic solvent is preferred for high stability.Lipases play a crucial role in digestion as well as the transportation and processing of dietary lipid substrates by catalyzing the hydrolysis of ester bonds in lipid substrates. The lipase from Aspergillus oryzae (AOL), a multipurpose biocatalyst can be used in the kinetic resolution of a biotin intermediate lactone,α-lipoic acid, and 1-phenylethanol. Additionally, it has the ability to stereoselectively catalyze the hydrolysis of ethyl 2-(4-hydroxyphenoxy) propanoate and its analogs, which are key intermediates in the production of aryloxyphenoxypropionate herbicides. AOL serves as a promising biocatalyst in the esterification of a series of short-chain acids and alcohols to produce flavor esters. It can be used to catalyze the esterification of lauric acid with aromatic alcohol-benzyl alcohol.

Uwaga dotycząca przygotowania

Produced by submerged fermentation of a genetically modified Aspergillus oryzae microorganism

Informacje prawne

A product of Novozyme Corp.
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enzym

Numer produktu
Opis
Cennik

Piktogramy

Health hazard

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Resp. Sens. 1

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Purification and characterization of lipase produced by Aspergillus oryzae CJLU-31 isolated from waste cooking oily soil
Zhou J, et al.
American Journal of Food Technology, 7(10), 596-608 (2012)
E E van Haaften et al.
Acta biomaterialia, 92, 48-59 (2019-05-21)
To maintain functionality during in situ vascular regeneration, the rate of implant degradation should be closely balanced by neo-tissue formation. It is unknown, however, how the implant's functionality is affected by the degradation of the polymers it is composed of.
Three-phase slug flow in microchips can provide beneficial reaction conditions for enzyme liquid-liquid reactions
Cech J, et al.
Biomicrofluidics, 7(5), 054103-054103 (2013)
Dzhuliya Dzhonova et al.
PloS one, 13(8), e0203409-e0203409 (2018-08-31)
Local drug delivery systems that adjust the release of immunosuppressive drug in response to the nature and intensity of inflammation represent a promising approach to reduce systemic immunosuppression and its side effects in allotransplantation. Here we aimed to demonstrate that
Jing Li et al.
Materials today. Communications, 24, 101197-101197 (2020-08-25)
The low cost lipase derived from Thermomyces lanugionous was chosen to conjugate with Fe3O4 nanoparitcles as a magnetic responsive lipase (MRL) biocatalyst. The structure of MRL was observed by atomic force microscopy (AFM). The Fourier transform infrared (FTIR) spectroscopy analysis

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