GE29-0491-04

HiTrap^® MabSelect SuRe^™

Cytiva 29-0491-04, pack of 1 mL

• Kod UNSPSC: 23151816
• NACRES: NA.56

Właściwości

• ligand: alkali-stabilized protein A-derived (E. coli)
• opakowanie: pack of 1 mL
• producent / nazwa handlowa: Cytiva 29-0491-04
• dostępność: not available in North America
• warunki przechowywania: (20% Ethanol); (20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)
• Parametry: 42 psi
• wymiary złoża: 7 mm × 25 mm
• objętość złoża: 1 mL
• kolumna śr. wewn.: 7 mm
• Matryca: highly cross-linked agarose
• śr. rozm. cząst.: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-13.7
• Zakres roboczy: 3-12
• pojemność: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• przydatność: suitable for bioprocess medium
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allows cleaning-in-place (CIP).

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Cechy i korzyści

• Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
• Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.

Przechowywanie i stabilność

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Inne uwagi

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informacje prawne

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.

HiTrap is a registered trademark of Cytiva

MabSelect SuRe is a trademark of Cytiva

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H226
• Zwroty wskazujące środki ostrożności: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Kod klasy składowania: 3 - Flammable liquids