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GE17-5199-01

MabSelect

Cytiva 17-5199-01, pack of 25 mL

Synonim(y):

MabSelect Column

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About This Item

Kod UNSPSC:
23151817
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

opakowanie

pack of 25 mL

producent / nazwa handlowa

Cytiva 17-5199-01

macierz

highly cross-linked agarose

śr. rozm. cząst.

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning in place

2-12

working range

3-10

pojemność

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

przydatność

suitable for bioprocess medium

temp. przechowywania

2-8°C

Opis ogólny

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
MabSelect is a protein A affinity medium designed for capturing MAbs from large sample volumes.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Zastosowanie

MabSelect is a BioProcess affinity medium for capturing monoclonal antibodies (MAbs) from large sample volumes. MabSelect is developed for cost-effective high throughput processing of large amounts of feed. This is the reason why MabSelect has been designed-in to several new and second-generation processes for the production of therapeutic MAbs.

The medium is based on a highly cross-linked Agarose matrix with a recombinant Protein A ligand. This ligand is engineered to favor an oriented coupling resulting in an affinity medium with enhanced binding capacity for IgG. The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool. MabSelect is optimized for high capacity, throughput and to deliver a product pool that is high in purity and yield.

The recombinant Protein A ligand is expressed in E. coli and is free of components of mammalian origin. The specificity of binding to the Fc region of IgG is similar to that of native Protein A giving a high purification factor in a single step. In addition, the high capacity and the novel base matrix make MabSelect an excellent choice for purification of MAbs at process scale.

MabSelect is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, MabSelect meets industrial demands with security of supply and comprehensive technical and regulatory support.

Cechy i korzyści

  • High-fLow Agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day.
  • Oriented coupling of the ligand and optimized matrix gives high dynamic binding capacity which reduces media volume requirements
  • Low non-specific binding resulting in Low levels of impurities in the product eluate pool
  • BioProcess medium supported for industrial applications and well-established in approved processes.

Przechowywanie i stabilność

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Komentarz do analizy

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informacje prawne

MabSelect is a trademark of Cytiva
This page may contain text that has been machine translated.

Piktogramy

Flame

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Kod klasy składowania

3 - Flammable liquids


Certyfikaty analizy (CoA)

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Produkty

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Zobacz wszystko

Protokoły

This page shows how to pack Tricorn 10/100 columns with MabSelect or MabSelect SuRe from GE Healthcare.

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Ta strona pokazuje, jak konwertować między przepływem liniowym a przepływem objętościowym w chromatografii powinowactwa.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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