GE28-9782-44

HiScreen^™ Ni Fast Flow

Cytiva 28-9782-44

• Kod UNSPSC: 13111000
• NACRES: NA.56

Właściwości

• opakowanie: pkg of 1 ea
• producent / nazwa handlowa: Cytiva 28-9782-44
• Parametry: 1.5 bar (22 psi) (Over the Packed Bed During Operation); 22 psi
• wymiary złoża: 7.7 mm × 100 mm
• objętość złoża: 4.7 mL
• kolumna śr. wewn.: 7.7 mm
• macierz: 6% cross-linked agarose
• wielkość cząstki: 45-165 μm
• średnia średnica: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium.)
• pojemność: ~40 mg binding capacity(histidine-tagged protein)
• przydatność: suitable for bioprocess medium

Opis

Opis ogólny

HiScreen^™ columns prepacked with Ni Sepharose^™ 6 Fast FLow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).

Zastosowanie

HiScreen^™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen^™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The chromatography media used in HiScreen^™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen^™ Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose^™ 6 Fast FLow which has high binding capacities for
histidine-tagged Proteins and minimal Ni2+ leakage.

Cechy i korzyści

• High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+.
• Prepacked, ready-to-use columns for convenient process development.
• Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
• Easy connection in series to achieve 20 cm bed height.
• Small bed volume gives fast results and minimal sample/buffer consumption
• Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

Przechowywanie i stabilność

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Komentarz do analizy

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informacje prawne

HiScreen is a trademark of Cytiva

Sepharose is a trademark of Cytiva

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS09
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H226
• Zwroty wskazujące środki ostrożności: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Kod klasy składowania: 3 - Flammable liquids