G8404

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

• Synonim(y): Entner-Doudoroff enzyme, G6PD, G6PDH, NADP glucose 6-phosphate dehydrogenase, G-6-P-DH
• Numer CAS: 9001-40-5
• Numer EC enzymu: 1.1.1.49 (BRENDA, IUBMB)
• Numer MDL: MFCD00081656
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: bacterial (Leuconostoc mesenteroides)
• Poziom jakości: 300
• rekombinowane: expressed in E. coli
• Formularz: ammonium sulfate suspension
• aktywność właściwa: ≥550 units/mg protein (biuret)
• masa cząsteczkowa: 128 kDa
• warunki przechowywania: (Tightly closed)
• metody: cell culture | mammalian: suitable
• numer dostępu UniProt: UPI0021A4D550
• obecność zanieczyszczeń: creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).G6PD is a dimer and consists of a single active site at each subunit.

Research area: Cell Signaling

Zastosowanie

Glucose-6-phosphate dehydrogenase has been used:

• to test ketose reductase activity in developing maize endosperm.
• to determine the levels of mannose in coronary heart disease patient-derived serum
• to study its activity on extracellular polymeric substance (EPS) extract to determine cell lysis through sonication
• to determine the glucose uptake in cultured human muscle satellite cells

Działania biochem./fizjol.

G6PD provides all cells with reducing power as nicotinamide adenine dinucleotide phosphate (NADPH) which enables the cells to balance oxidative stress. Mutations in the G6PD gene are associated with X-linked G6PD deficiency, a hereditary genetic defect such as neonatal jaundice and acute hemolytic anemia.

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Definicja jednostki

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Postać fizyczna

Suspension in 3.2 M (NH₄)₂SO₄ containing 50 mM Tris and 1 mM MgCl₂, pH 7.5

Inne uwagi

The volume is lot specific and can be calculated using the data:
For example, lot 0000114274
Units per mg Protein = 846
Mg Protein per mL - 9
Units per mL = 7614
G8404-1000U = approx 132 ul

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type ABEK (EN14387) respirator filter