G5760

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret

• Synonim(y): G-6-P-DH
• Numer CAS: 9001-40-5
• Numer EC enzymu: 1.1.1.49 (BRENDA, IUBMB)
• Numer WE: 232-602-6
• Numer MDL: MFCD00081656
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: bacterial (Leuconostoc mesenteroides)
• Poziom jakości: 200
• typ: Type XXIII
• Postać: ammonium sulfate suspension
• aktywność właściwa: 550-1,100 units/mg protein (biuret)
• masa cząsteczkowa: 54 kDa
• stężenie: ≥2.0 mg/mL Biuret
• obecność zanieczyszczeń: 6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%; PGI ≤0.01%
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is found in the cytoplasm of all cells. The G6PD enzyme is encoded by the Gd gene, located on the long arm of the X chromosome.Glucose-6-phosphate Dehydrogenase (G6PD) from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺). It corresponds to a molecular weight of 54 kDa and exists as a homodimer.

Zastosowanie

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used:

• as a component of reaction mixture for assaying mannose- and glucose-6-phosphate activity
• as a component of reaction mixture in the nevirapine inhibition studies in human hepatic microsomes
• in the glucose-phosphorylating activity of chloroplast extracts
• as a model to test the effect of seed protein fractions on enzyme protection during dehydration.
• in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.

Działania biochem./fizjol.

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase (G6PD) is a housekeeping enzyme that protects cells from damage caused by reactive oxygen species by supplying substrates that help prevent oxidative harm. G6PD from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺) as the first step in the pentose phosphate pathway. Lysine 21 in the Glucose-6-phosphate Dehydrogenase (G6PD) is crucial for binding NAD⁺. G6PD uses NAD⁺ or NADP⁺ based on the catabolic or anabolic metabolic pathway.G6PD deficiency can lead to acute hemolysis, neonatal jaundice, or severe chronic non-spherocytic hemolytic anemia.

Definicja jednostki

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Postać fizyczna

Suspension in 2.7 M (NH₄)₂SO₄ solution containing 42 mM Tris and 0.8 mM MgCl₂

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)