10165875001

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

from Leuconostoc mesenteroides

• Synonim(y): G6P-DH, Glucose-6-Phosephate Dehydrogenase
• Numer EC enzymu: 1.1.1.49 (BRENDA, IUBMB)
• Kod UNSPSC: 12352204

Właściwości

• pochodzenie biologiczne: bacterial (Leuconostoc mesenteroides)
• Poziom jakości: 100
• Formularz: solution; suspension
• aktywność właściwa: ~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)
• masa cząsteczkowa: dimer 110 kDa
• opakowanie: pkg of 1 mL (1,000 U)
• producent / nazwa handlowa: Roche
• stężenie: ≥0.1-1.0 % (w/w)
• metody: activity assay: suitable
• kolor: white
• optymalne pH: 7.0-8.5(maximal activity at 7.8)
• rozpuszczalność: water: miscible
• numer dostępu NCBI: AAA25265
• numer dostępu UniProt: P11411
• Zastosowanie: life science and biopharma
• obecność zanieczyszczeń: 6-PGDH <0.001%; CK <0.001%; GR <0.01%; HK <0.05%; NADH oxidase <0.02%; PGI <0.01%
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.1

Specyficzność

Specificity: At pH 7.8, 25 °C: G6P-DH from Leuconostoc (LG6PDH) is highly specific for D-glucose-6-phosphate (K_m = 36 μM, NADP as coenzyme; 64 μM, NAD as coenzyme), but will use either NADP (K_m = 7.4 μM; relative rate 1.0) or NAD (K_m = 115 μM; relative rate 1.8) as coenzyme.
LG6P-DH does not react with fructose-6-phosphate, fructose-1,6-biphosphate, glucose-1-phosphate or ribose-1-phosphate. LG6P-DG will oxidize 2-deoxy-glucose-6-phosphate with NADP, but not with NAD as coenzyme. There is a slow reaction with D-glucose.
Heat inactivation: The ammonium sulfate suspension is not inactivated when heated to temperatures ≤ 50 °C for 10 minutes. At temperatures > 60 °C the enzyme is rapidly inactivated.

Zastosowanie

Component of cofactor recycling systems for NADPH.

It was used in enzymatic determination of C6 phosphorylation of glycogen.

Jakość

Contaminants: <0.001% CK, <0.01% GR and PGI each, <0.02% “NADH oxidase”, <0.05% HK, <0.001% 6-PGDH

Definicja jednostki

Unit Conversion: One unit (U) [+25 °C] ≈ 1.18 U [+30 °C] ≈ 1.45 U [+37 °C], all with NAD as coenzyme

Unit Definition: One unit (U) LG6P-DH oxidizes 1 mol of glucose-6-phosphate and reduces 1 mol of NAD in 1 minute at +25 °C and pH 7.8.

Postać fizyczna

Solution of 1,000 U in 1 ml 3.2 M ammonium sulfate, pH approximately 6

Uwaga dotycząca przygotowania

Activator: HCO^3- (≤ 0.3 M) activates slightly.

Komentarz do analizy

Absorbance of purified enzyme: 1.15 (1 mg enzyme/ml, 280.5 nm)

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash