P3397

Phosphoglucomutase from rabbit muscle

ammonium sulfate suspension, ≥100 units/mg protein

• Synonim(y): PGM, α-D-Glucose-1,6-bisphosphatase, α-D-Glucose-1-phosphate phosphotransferase
• Numer CAS: 9001-81-4
• Numer EC enzymu: 5.4.2.2 (BRENDA, IUBMB)
• Numer MDL: MFCD00131886
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: rabbit muscle
• Poziom jakości: 300
• opis: ammonium sulfate suspension, >=100 units/mg protein
• Formularz: ammonium sulfate suspension
• aktywność właściwa: ≥100 units/mg protein
• warunki przechowywania: (Tightly closed)
• metody: activity assay: suitable
• kolor: white to light yellow
• obecność zanieczyszczeń: lactic dehydrogenase ≤0.5%; phosphoglucose isomerase ≤0.01%; pyruvate kinase ≤0.05%
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Research area: Cell Signaling

Phosphoglucomutase (PGM), a conserved enzyme, is abundantly found in animals, plants, and microorganisms. It is distributed in almost all tissues.

Zastosowanie

Phosphoglycomutase has been used:

• to study glycogenesis and phosphoglycomutase deficiency in humans
• in phosphoglucomutase assays to study metabolic regulation
• for assay A to assay enzymes closely linked to glycogen metabolism in transformed BL21(DE3)C43 cells
• in sucrose synthase (SuSy) andADPglucose pyrophosphorylase (AGPase) assays(3)
• in enzyme activity assay for coupling the reduction of nicotinamide adenine dinucleotide phosphate (NADP⁺)to determine glucose-1-phosphate from sucrose and inorganic phosphate (Pi)

Działania biochem./fizjol.

Phosphoglucomutase(PGM) enzyme plays a vital role in glycolysis and gluconeogenesis. It is involved in glycogen and trehalose metabolism in insects. PGM participates in the development of plants and some microorganisms. It is essential for proteins, lipids, and nucleic acid metabolism and is crucial for the development of plants because glucose-6-phosphate is a crucial central metabolite. PGM catalyzes the interconversion of glucose-6-phosphate (G-6-P)and glucose-1-phosphate (G-1-P).

Definicja jednostki

One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.

Postać fizyczna

Crystalline suspension in 3.2 M (NH₄)₂SO₄, pH 6.0 containing 0.01% EDTA

Komentarz do analizy

Protein determined by biuret.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)