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P5381
Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)
Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)
Synonim(y):
D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
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About This Item
Polecane produkty
pochodzenie biologiczne
bakers yeast
typ
Type III
Formularz
ammonium sulfate suspension
aktywność właściwa
≥400 units/mg protein (biuret)
masa cząsteczkowa
tetramer 119,500 Da±600
stężenie
≥3 mg/mL protein Biuret
metody
activity assay: suitable
kolor
white
numer dostępu Protein ID
numer dostępu UniProt
temp. przechowywania
2-8°C
informacje o genach
yeast ... PGI1(852495)
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Opis ogólny
Research area: Cell Signaling
Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.
Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.
Zastosowanie
Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:
- to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.
- as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.
- to convert fructose to glucose while evaluating fructose content in kudzu roots.
Działania biochem./fizjol.
Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP). It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.
Definicja jednostki
One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.
Postać fizyczna
Crystalline suspension in 2.6 M (NH4)2SO4 containing 0.1 mM magnesium acetate, pH 7.0
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves
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S L Lowe et al.
The Journal of biological chemistry, 250(1), 94-99 (1975-01-10)
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Produkty
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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