G4134

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

• Synonim(y): G-6-P-DH, Zwischenferment
• Numer CAS: 9001-40-5
• Numer EC enzymu: 1.1.1.49 (BRENDA, IUBMB)
• Numer WE: 232-602-6
• Numer MDL: MFCD00081656
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• typ: Type IX
• Poziom jakości: 200
• Formularz: lyophilized powder
• aktywność właściwa: 200-400 units/mg protein (modified Warburg-Christian)
• masa cząsteczkowa: 128 kDa
• Zawartość β-NADP i β-NADPH: ≤10 mmol/mol
• Zastosowanie: agriculture
• Warunki transportu: dry ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.

Zastosowanie

Glucose-6-phosphate dehydrogenase is used:

• To test ketose reductase activity in developing maize endosperm.
• For recycling microassay of β-NADP and β-NADPH.
• To measure the intracellular levels of NADPH and total NADP.
• To measure the nicotinamide adenine dinucleotide (NAD) kinase kinetic assay activity.

Działania biochem./fizjol.

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase catalyzes the rate-limiting step in the pentose phosphate pathway. Its function involves the conversion of glucose-6-phosphate to 6-phosphogluconolacetone while generating NADPH, which is essential for the regeneration of glutathione The glutathione system utilizes nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) to effectively eliminate excess hydrogen peroxide. Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in regulating cell growth and survival. Their levels are higher in cells undergoing normal and neoplastic growth. Increased glucose-6-phosphate dehydrogenase activity plays a pivotal role in preventing reactive oxygen species mediated cell death. Glucose-6-phosphate dehydrogenase is over expressed in several cancers whereas its activity is reduced in hyperglycemia. A deficiency in glucose-6-phosphate dehydrogenase causes hemolysis.

Definicja jednostki

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Postać fizyczna

Lyophilized powder essentially sulfate-free, containing approx. 20% sodium citrate

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)