L8

95102434, rat muscle, Myoblast

• Kod UNSPSC: 41106514

Właściwości

• product name: L8, 95102434
• pochodzenie biologiczne: rat muscle
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Myoblast
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Rat Skeletal muscle myoblast

Opis linii komórkowej

The myogenic cell line L8 was isolated by D Yaffe in 1969 by selective serial passage of myoblasts from primary rat skeletal muscle cell cultures (new born, non-in-bred Wistar rats). Subsequently they were recloned by H Blau. L8 cells differentiate into cross-striated myotubes by fusion and express several muscle-specific proteins. The myoblast population will be depleted if cells are allowed to become confluent before sub-culturing. Therefore, sub-cultivation before confluency and periodical re-cloning is necessary. The preparation of a frozen stock at early passage is recommended.

Zastosowanie

Study of muscle differentiation, expression and analysis of muscle-specific proteins

pożywka hodowlana

DMEM : Medium 199 (4:1) + 2mM Glutamine + 1% CEE (Chick Embryo extract (Seralab)) + 10% Horse Serum (HS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 cells to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C; sub-cultivate before confluency and reclone periodically.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This page may contain text that has been machine translated.