BLO-11

90112702

• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: mouse muscle
• tryb wzrostu: Adherent
• kariotyp: 2n = 40
• morfologia: Fibroblast-like
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse Moblo/y muscle hereditary emphysema

Opis linii komórkowej

Established from the abdominal wall muscle of a 30 day old male Blotchy mouse (Mo (blo/y)) with hereditary emphysema. The cells are deficient in lysyl oxidase activity and accumulate high levels of intracellular copper. NOR-10 (Sigma Catalogue number. 90112701) was isolated from the abdominal wall muscle of a normal litter mate of the mouse from which BLO-11 was derived, thus it can serve as a matched control for studies with BLO fibroblasts.

Zastosowanie

Genetic studies

pożywka hodowlana

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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