SP2/O-Ag14 Cell Line murine

Non-producing hybridoma, 85072401

• Synonim(y): Mouse Cell Line
• Kod UNSPSC: 12352207

Właściwości

• pochodzenie biologiczne: mouse spleen
• tryb wzrostu: Suspension
• kariotyp: Not specified
• morfologia: Not specified
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse x Mouse myeloma, non-producing

Opis linii komórkowej

Sp2/0-Ag14 is a non-Ig-secreting or synthesising line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Resistant to 8-azaguanine at 20ug/ml and does not survive in HAT containing media. HAT sensitive mouse myeloma hybrid suitable for use as a hybridoma fusion partner - in this sense, functionally it is a myeloma cell line and often referred to as such in the literature.

Zastosowanie

Fusion partner

pożywka hodowlana

RPMI 1640 or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. HAT sensitive.

Inne uwagi

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