HeLa (AC-free)

8011102, human cervix, Spherical

• Kod UNSPSC: 41106514

Właściwości

• product name: HeLa (AC-free), 08011102
• pochodzenie biologiczne: human cervix
• tryb wzrostu: Aggregates in suspension
• kariotyp: 2n = 46, aneuploid
• morfologia: Spherical
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human cervical carcinoma, serum-free, animal component (AC) free

Opis linii komórkowej

HeLa cell line (Sigma catalogue no. 93021013) adapted to grow in animal component free medium (EX-CELL HeLa Serum-Free Medium for HeLa Cells Sigma cat no 14591C).

Zastosowanie

Virus studies

pożywka hodowlana

EX-CELL HeLa Serum-Free Medium (Sigma cat no 14591C)+ 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

Rutyna subkultury

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

Inne uwagi

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