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Key Documents
C3389
Acetylcholinesterase from Electrophorus electricus (electric eel)
Type VI-S, lyophilized powder, 200-1,000 units/mg protein
Synonim(y):
AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
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About This Item
Polecane produkty
typ
Type VI-S
Poziom jakości
Postać
lyophilized powder
aktywność właściwa
200-1,000 units/mg protein
masa cząsteczkowa
280 kDa
skład
Protein, ≥45% biuret
rozpuszczalność
20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)
Zastosowanie
diagnostic assay manufacturing
temp. przechowywania
−20°C
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Opis ogólny
Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Zastosowanie
The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity. The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.
Działania biochem./fizjol.
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
Definicja jednostki
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
Postać fizyczna
Lyophilized powder containing Tris buffer salts
Uwaga dotycząca przygotowania
This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution.
Komentarz do analizy
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
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Dan Du et al.
Analytical and bioanalytical chemistry, 387(3), 1059-1065 (2006-12-23)
A simple method has been devised for immobilization of acetylcholinesterase (AChE)--covalent bonding to a multiwall carbon nanotube (MWNT)--cross-linked chitosan composite (CMC)-and a sensitive amperometric sensor for rapid detection of acetylthiocholine (ATCl) has been based on this. Fourier-transform infrared spectroscopy proved
Melissa Salinas et al.
Plants (Basel, Switzerland), 11(9) (2022-05-15)
The aim of this study was to extract and identify the chemical compounds of Diplosthephium juniperinum essential oil (EO) from Ecuador and to assess its anticholinesterase and antioxidant properties. The EO chemical composition was determined by GC-MS. A total of
Jingming Gong et al.
Biosensors & bioelectronics, 24(7), 2285-2288 (2008-12-30)
We developed a simple strategy for designing a highly sensitive electrochemical biosensor for organophosphate pesticides (OPs) based on acetylcholinesterase (AChE) immobilized onto Au nanoparticles-polypyrrole nanowires composite film modifid glassy carbon electrode (labeled as AChE-Au-PPy/GCE). Where, the generated Au nanoparticles (AuNPs)
Gabriela Villalta et al.
Plants (Basel, Switzerland), 10(6) (2021-07-03)
The essential oil (EO) of Salvia leucantha Cav. was isolated by steam distillation of the aerial parts collected in the South of Ecuador. Its physical properties were evaluated and the chemical composition of the oil was determined by GC-MS and
Makar Makarian et al.
Journal of molecular structure, 1247 (2022-03-01)
In an effort to develop new therapeutic agents to treat Alzheimer's disease, a series of donepezil-based analogs were designed, synthesized using an environmentally friendly route, and biologically evaluated for their inhibitory activity against electric eel acetylcholinesterase (AChE) enzyme. In vitro
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