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Key Documents
C2888
Acetylcholinesterase from Electrophorus electricus (electric eel)
Type V-S, lyophilized powder, ≥1,000 units/mg protein
Synonim(y):
AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
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About This Item
Polecane produkty
pochodzenie biologiczne
Electrophorus electricus
Poziom jakości
typ
Type V-S
Postać
lyophilized powder
aktywność właściwa
≥1,000 units/mg protein
masa cząsteczkowa
280 kDa
skład
Protein, ≥45% biuret
pI
~5.3
rozpuszczalność
Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
temp. przechowywania
−20°C
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Opis ogólny
Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Zastosowanie
Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
- in acetylcholinesterase activity assay
- to study its effects on Xenopus embryonic hippocampal cells
- to study the effect of acetylcholine inhibitors on development and plasticity
Działania biochem./fizjol.
Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
Przestroga
For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.
Definicja jednostki
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
Postać fizyczna
Lyophilized powder containing Tris buffer salts
Komentarz do analizy
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
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