D8130

5,5′-Dithiobis(2-nitrobenzoic acid)

≥98%, BioReagent, suitable for determination of sulfhydryl groups

• Synonim(y): 3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent
• Wzór liniowy: [-SC₆H₃(NO₂)CO₂H]₂
• Numer CAS: 69-78-3
• Masa cząsteczkowa: 396.35
• Beilstein: 1896821
• Numer WE: 200-714-4
• Numer MDL: MFCD00007140
• Kod UNSPSC: 12352106
• Identyfikator substancji w PubChem: 24894189
• NACRES: NA.26

Właściwości

• linia produktu: BioReagent
• Poziom jakości: 200
• Próba: ≥98%
• Postać: powder
• mp: 240-245 °C (dec.) (lit.)
• przydatność: suitable for determination of sulfhydryl groups
• ciąg SMILES: OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O
• InChI: 1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
• Klucz InChI: KIUMMUBSPKGMOY-UHFFFAOYSA-N

Opis

Opis ogólny

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS^-) reacts with Ellman′s reagent (DTNB^2-), to produce 2-nitro-5-thiobenzoate anion (TNB^2-) and one mixed disulfide (R-S-TNB^-). TNB^2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.

Zastosowanie

Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues. It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves