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Anti-Mouse IgG (whole molecule)−Atto 488 antibody produced in goat
~1 mg/mL
Synonim(y):
Atto 488 przeciw mysim IgG, Kozie anty-Mysie IgG
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.46
Polecane produkty
pochodzenie biologiczne
goat
białko sprzężone
Atto 488 conjugate
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
secondary antibodies
klon
polyclonal
Formularz
liquid
zawiera
50% glycerol as stabilizer
reaktywność gatunkowa
mouse
stężenie
~1 mg/mL
metody
immunocytochemistry: 1:500
immunofluorescence: 1:400
fluorescencja
λex 500 nm; λem 522 nm in PBS
Warunki transportu
wet ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
Powiązane kategorie
Opis ogólny
IgG is the most abundant antibody in human serum. It is secreted by B cells andis found in blood. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, that is linked together by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Anti-Mouse IgG (whole molecule)-Atto 488 antibody is specific for mouse IgG. The product is purified from goat anti-mouse IgG antiserum to remove all goat serum proteins, including immunoglobulins that do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity.
Immunogen
purified mouse IgG.
Zastosowanie
Anti-Mouse IgG (whole molecule)?Atto 488 antibody produced in goat has been used:
- in immunofluorescence confocal microscopic assay for p65 localization
- in immunohistochemical analysis
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Działania biochem./fizjol.
IgG facilitates transplacental transport and participates in the secondary immune response. It has the longest serum half life. It might be linked to allergy. IgG initiates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC).
Postać fizyczna
Solution in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, andcontaining 5 mM sodium azide as a preservative.
Komentarz do analizy
unconjugated dye ≤5% of total fluorescence
Informacje prawne
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
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Produkty
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
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