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70746

Millipore

Benzonase® Nuclease

Purity > 99%

Synonim(y):

Endonuclease from Serratia marcescens

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About This Item

Numer CAS:
9025-65-4
Numer EC enzymu:
3.1.30.2 (BRENDA, IUBMB)
Numer MDL:
MFCD00131010
Kod UNSPSC:
12352202
NACRES:
NA.54

product name

Benzonase® Nuclease, Purity > 90%,

pochodzenie biologiczne

Serratia marcescens

Poziom jakości

300

rekombinowane

expressed in E. coli

Próba

>90% (SDS-PAGE)

Postać

buffered aqueous glycerol solution

producent / nazwa handlowa

Novagen®

warunki przechowywania

OK to freeze

stężenie

25-29 units/μL

Zastosowanie

research use

Warunki transportu

wet ice

temp. przechowywania

−20°C

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Opis ogólny

Benzonase® Nuclease is a geneticallyengineered endonuclease from Serratiamarcescens. It degrades all forms of DNA andRNA (single stranded, double stranded, linearand circular) while having no proteolyticactivity. It is effective over a wide range ofconditions and possesses an exceptionallyhigh specific activity. The enzyme completelydigests nucleic acids to 5′-monophosphateterminated oligonucleotides 2 to 5 bases inlength (below the hybridization limit), whichis ideal for removal of nucleic acids fromrecombinant proteins, enabling compliancewith FDA guidelines for nucleic acidcontamination. The ability of Benzonase torapidly hydrolyze nucleic acids makes theenzyme an excellent choice for viscosityreduction to reduce processing time andincrease yields of protein. For example, theenzyme is compatible with BugBuster andPopCulture Protein Extraction Reagentsand can therefore be added along with thesereagents to eliminate viscosity and removenucleic acids from E. coli extracts.

The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by >150 mM monovalentcations, >100 mM phosphate, >100 mMammonium sulfate, or >100 mM guanidine HCl.

Benzonase Nuclease is available inultrapure (>99% by SDS-PAGE) and pure(>90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 106 U/mg protein. The >99% puritygrade is tested for endotoxins and contains<0.25 EU/1000 units. The product is suppliedin 50% glycerol.Store at -20°C.

Total endotoxin:<0.25 EU/1,000 units.Purity: >90% by SDS-PAGE

Zastosowanie

Used for the removal of nucleic acid from protein samples.

Działania biochem./fizjol.

Digests native or heat-denatured DNA and RNA.

Ostrzeżenie

Toxicity: Standard Handling (A)

Definicja jednostki

One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.

Informacje prawne

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Frederic D Schramm et al.
PLoS genetics, 13(12), e1007148-e1007148 (2017-12-28)
Hsp70 chaperones are well known for their important functions in maintaining protein homeostasis during thermal stress conditions. In many bacteria the Hsp70 homolog DnaK is also required for growth in the absence of stress. The molecular reasons underlying Hsp70 essentiality
Asaf Poran et al.
Cell reports. Medicine, 1(8), 100141-100141 (2020-12-10)
T cells use highly diverse receptors (TCRs) to identify tumor cells presenting neoantigens arising from genetic mutations and establish anti-tumor activity. Immunotherapy harnessing neoantigen-specific T cells to target tumors has emerged as a promising clinical approach. To assess whether a comprehensive
Alexander Reim et al.
Nature communications, 11(1), 3019-3019 (2020-06-17)
Transcription factors (TFs) regulate target genes by specific interactions with DNA sequences. Detecting and understanding these interactions at the molecular level is of fundamental importance in biological and clinical contexts. Crosslinking mass spectrometry is a powerful tool to assist the
David M Kern et al.
Methods in enzymology, 653, 207-235 (2021-06-09)
The SARS-CoV-2 3a protein is a putative ion channel implicated in virus life cycle and pathogenesis. We recently expressed, purified, and reconstituted 3a into lipid nanodiscs to solve its structure by cryo-EM to 2.1Å resolution. In this chapter, we describe
Jonathan Elegheert et al.
Nature protocols, 13(12), 2991-3017 (2018-11-21)
Structural, biochemical and biophysical studies of eukaryotic soluble and membrane proteins require their production in milligram quantities. Although large-scale protein expression strategies based on transient or stable transfection of mammalian cells are well established, they are associated with high consumable
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Produkty

Benzonase® Nuclease Q&A

This page lists nine frequently asked questions and answers about Benzonase® Nuclease.

Pytania i odpowiedzi dotyczące nukleazy Benzonase®

Na tej stronie znajduje się lista dziewięciu często zadawanych pytań i odpowiedzi dotyczących Benzonase® Nuclease.

Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

The combination of BugBuster and Lysonase greatly enhances the release of soluble proteins from Gram-positive bacteria.

Usuwanie DNA/RNA w produkcji wirusów

Endonukleaza Benzonase® skutecznie usuwa zanieczyszczenia kwasami nukleinowymi z produkcji wirusów, co ma kluczowe znaczenie dla terapii komórkowych i genowych oraz szczepionek.

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