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70922

Millipore

BugBuster® HT Protein Extraction Reagent

Synonim(y):

Cell lysis reagent, Protein extraction buffer

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About This Item

Kod UNSPSC:
41106511
NACRES:
NA.77

Postać

liquid

Poziom jakości

100

producent / nazwa handlowa

Novagen®

warunki przechowywania

OK to freeze
avoid repeated freeze/thaw cycles

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Powiązane kategorie

Opis ogólny

BugBuster HT Protein Extraction Reagent combines BugBuster Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Zastosowanie

BugBuster® HT Protein Extraction Reagent has been used:
  • to lyse the Escherichia coli cell pellets following centrifugation for protein expression and immunoblotting
  • for protein extraction from bacterial cell pellet
  • used to lyse the E. coli cell pellets for the expression and purification of dMIC60 (mitofilin) protein

Cechy i korzyści

  • Fast protein extraction and nucleic acid degradation
  • Ideal for processing various samples of different volumes
  • Increased extraction is ensured by the addition of rLysozyme solution

Ostrzeżenie

Toxicity: Standard Handling (A)

Informacje prawne

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Chiara Pedicone et al.
iScience, 25(4), 104170-104170 (2022-04-26)
Here, we describe the use of artificial intelligence to identify novel agonists of the SH2-containing 5' inositol phosphatase 1 (SHIP1). One of the compounds, K306, represents the most potent agonist identified to date. We find that K306 exhibits selectivity for

Produkty

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster® Plus Lysonase™ Kit: Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria

Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit

Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

The combination of BugBuster and Lysonase greatly enhances the release of soluble proteins from Gram-positive bacteria.

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