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Merck

IPTG-RO

Roche

Isopropyl-β-D-thiogalactoside

mol wt 238.3, pkg of 1 g (10724815001), pkg of 5 g (11411446001)

Synonim(y):

Isopropyl β-D-1-thiogalactopyranoside, iptg, IPTG, Isopropyl β-D-thiogalactoside

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About This Item

Wzór empiryczny (zapis Hilla):
C9H18O5S
Numer CAS:
Masa cząsteczkowa:
238.30
Beilstein:
4631
Numer MDL:
Kod UNSPSC:
12352200
Identyfikator substancji w PubChem:

Formularz

powder

masa cząsteczkowa

238.3

opakowanie

pkg of 1 g (10724815001)
pkg of 5 g (11411446001)

producent / nazwa handlowa

Roche

Warunki transportu

wet ice

temp. przechowywania

2-8°C

ciąg SMILES

CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

Klucz InChI

BPHPUYQFMNQIOC-NXRLNHOXSA-N

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Opis ogólny

Isopropyl-β-D-thiogalactoside (IPTG) is a chemical analog of galactose that cannot be cleaved by β-galactosidase. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Zastosowanie

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Isopropyl-β-D-thiogalactoside (IPTG) is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. It has been used for the expression of recombinant genes in E. coli.

Inne uwagi

Non-metabolizable galactose analog.
For life science research only. Not for use in diagnostic procedures.
The amount of labeled DNA depends on:
  • amount of template DNA
  • purity of template DNA
  • conformation of template DNA
  • average size of labeled fragment

Charakterystyka techniczna

Assay Time: 50minutes
Specific Activity: The described standard assay will results in a specific activity of 1.8 x 109 dpm/μg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.

Uwaga dotycząca przygotowania

Sample Material
10 ng to 3μg template DNA, linearized and at least 100 to 200bp long
Working concentration: Final concentration should be 0.2 mM.
Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Sonja-Verena Albers et al.
Journal of bacteriology, 185(13), 3918-3925 (2003-06-19)
A large number of secretory proteins in the thermoacidophile Sulfolobus solfataricus are synthesized as a precursor with an unusual leader peptide that resembles bacterial type IV prepilin signal sequences. This set of proteins includes the flagellin subunit but also various
Louis Droogmans et al.
Nucleic acids research, 31(8), 2148-2156 (2003-04-12)
N1-methyladenosine (m1A) is found at position 58 in the T-loop of many tRNAs. In yeast, the formation of this modified nucleoside is catalyzed by the essential tRNA (m1A58) methyltransferase, a tetrameric enzyme that is composed of two types of subunits
L H Hansen et al.
Current microbiology, 36(6), 341-347 (1998-06-03)
The role of the Escherichia coli lacY gene product (the lactose permease) in the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) inducible promoters was studied in E. coli and P. fluorescens. This was done by comparing strains containing a lacIPOZYA chromosomal insert with

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