11284932001
Roche
DNase I
from bovine pancreas
Synonim(y):
Dnase
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About This Item
pochodzenie biologiczne
bovine pancreas
Poziom jakości
Postać
lyophilized
opakowanie
pkg of 100 mg
producent / nazwa handlowa
Roche
metody
cell culture | mammalian: suitable
temp. przechowywania
2-8°C
Opis ogólny
DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin.
Zastosowanie
DNase I has been used for tissue dissociation in conjunction with collagenases.
Działania biochem./fizjol.
During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.
Cechy i korzyści
- Lyophilized
- Filtered through 0.2 μm pore size membrane before lyophilization
- Glycoprotein and double-strand-specific endonuclease
Definicja jednostki
One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions.
Uwaga dotycząca przygotowania
Activator: Bivalent metal ions (Ca2+, Mg2+)
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.
Rekonstytucja
Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
This page may contain text that has been machine translated.
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1 - Skin Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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