T4330
Turbonuclease from Serratia marcescens
recombinant, expressed in E. coli
Synonim(y):
Endonuclease from Serratia marcescens
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About This Item
Numer CAS:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.54
Polecane produkty
pochodzenie biologiczne
Serratia marcescens
Poziom jakości
rekombinowane
expressed in E. coli
Postać
liquid
stężenie
≥200,000 units/mL
metody
DNA purification: suitable
przydatność
suitable for cell lysis
Zastosowanie
life science and biopharma
temp. przechowywania
−20°C
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Opis ogólny
Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.
Zastosowanie
Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies.
Turbonuclease has been used in a study to assess the TY3 gag3 spacer effect on intracellular condensation and uncoating.
Used for the removal of nucleic acid from protein samples.
Działania biochem./fizjol.
Endonuclease from Serratia marcescens is effective against both single- and double-stranded DNA and RNA. It mediates the digestion of the 3′ O—P bond resulting in oligonucleotides ending with 5′ monophosphate. The activity of this enzyme is known to be less affected by the reducing and chaotropic agents. It is highly stable at room temperature. This endonuclease eliminates the undesired nucleic acids in downstream processing.
Turbonuclease provides a nuclease treatment by reducing viscosity and degrading RNA, genomic DNA, baculovirus DNA, and unencapsidated vector DNA.
Digests native or heat-denatured DNA and RNA.
Definicja jednostki
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C.
Postać fizyczna
Supplied as a solution in 50 mM Tris-HCl, pH 8.0 and 50 mM NaCl
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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M D Miller et al.
Journal of molecular biology, 288(5), 975-987 (1999-05-18)
Serratia endonuclease is an important member of a class of magnesium dependent nucleases that are widely distributed in nature. Here, we describe the location and geometry of a magnesium-water cluster within the active site of this enzyme. The sole protein
Kristina Clemens et al.
Journal of virology, 85(7), 3055-3066 (2011-01-29)
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