69671

Thrombin, Restriction Grade

• Synonim(y): Thrombin, Restriction Grade
• Numer CAS: 9002-04-4
• Kod UNSPSC: 12352202
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: human plasma
• Poziom jakości: 400
• Postać: liquid
• producent / nazwa handlowa: Novagen^®
• warunki przechowywania: OK to freeze; avoid repeated freeze/thaw cycles
• metody: protein extraction: suitable
• przydatność: suitable for additive or modifier in the separation of proteins or peptides
• Zastosowanie: life science and biopharma
• Warunki transportu: wet ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Restriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.

Komponenty

•50 UThrombin

•1 ml10X Thrombin Cleavage Buffer

•2 ml1X Thrombin Dilution/Storage Buffer

•10 µgCleavage Control Protein

Ostrzeżenie

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Definicja jednostki

One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl₂, 50 µg fusion protein.

Uwaga dotycząca przygotowania

Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Informacje prawne

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1