M9004

リンゴ酸デヒドロゲナーゼ ウシ心臓由来

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

• 別名: L-リンゴ酸:NAD⁺オキシドレダクターゼ, MDH
• Enzyme Commission number: 1.1.1.37 (BRENDA, IUBMB)
• EC Number: 232-622-5
• MDL番号: MFCD00131567
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: bovine heart
• フォーム: ammonium sulfate suspension
• 比活性: 2000-4000 units/mg protein (modified Warburg-Christian)
• 分子量: 35 kDa
• 不純物: ≤0.01% Glutamic-Oxalacetic Transaminase; ≤0.01% Glutamic-Pyruvic Transaminase
• 保管温度: 2-8°C

詳細

詳細

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

アプリケーション

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.

単位の定義

1 unitは、pH 7.5、25°C、1分間で、1.0 μmolのオキサロ酢酸とβ-NADHを_L-リンゴ酸とβ-NADに変換します。

物理的形状

3 M (NH₄)₂SO₄ - 0.01 M KH₂PO₄溶液中に分散させた懸濁液(pH 7.3)

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)