C6357

カスパーゼ4 ヒト

recombinant, expressed in E. coli, 5,000 units/mg protein

• 別名: ICE(rel)II, Ich-2, TX
• Enzyme Commission number: 3.4.22.57 (BRENDA, IUBMB)
• MDL番号: MFCD03456356
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• リコンビナント: expressed in E. coli
• フォーム: solution
• 比活性: 5,000 units/mg protein
• 分子量: 30 kDa
• 溶解性: PBS: soluble
• UniProtアクセッション番号: P49662
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... CASP4(837)

詳細

詳細

The caspase 4 (CASP4) gene is mapped to human chromosome 11q21. Caspase 4 is a cysteine-aspartic protease, localized to the outer membrane of the endoplasmic reticulum (ER). The N-terminus of the enzyme contains a caspase recruitment domain (CARD).

生物化学的／生理学的作用

Caspase 4 is an inflammatory caspase associated with innate immune responses. It plays a key role in the endoplasmic reticulum (ER) stress-induced apoptosis. Caspase 4 mediates the fusion of phagosomes containing pathogens with the lysosome. It inhibits pathogen replication, development, and production of pro-inflammatory cytokines. Caspase 4 induces caspase activation and pyropoptotic death by binding to the intracellular lipopolysaccharide (LPS) of the Gram-negative bacterial outer membrane. This is mediated by the highly specific caspase recruitment domain (CARD).

Useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, as well as serving as positive controls in caspase activity assays and Western blot analysis.

単位の定義

1ユニットは、pH 7.2、37°Cで1時間あたりカスパーゼ基質であるWEHD-pNA 1 nmolをWEHDおよびp-ニトロアニリンに加水分解する量です。

物理的形状

Solution in 0.052% ammonium chloride, 0.158% Tris−HCl, and 0.76% sodium chloride

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable