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詳細
Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1 Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN Entrez Gene Name: actin, alpha 1, skeletal muscle Hu Entrez ID: 58, 71 (Related Antibodies: CP64) Mu Entrez ID: 11459 ;-; 11465 Rat Entrez ID: 29437 ;-; 287876
免疫原
Chicken
chicken cytoskeletal proteins
アプリケーション
Immunoblotting (1:5000-1:20000)
Immunofluorescence (1:10000, see application references)
Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
Immunofluorescence (1:10000, see application references)
Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
物理的形状
100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
再構成
Following initial thaw, aliquot ascites and freeze (-20°C).
アナリシスノート
Positive Control
All animal cell lines
All animal cell lines
その他情報
Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
11 - Combustible Solids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
CP01-1EA:
CP01-ML:
CP01-100UL:
US2CP01-1EA:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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