539134

Protease Inhibitor Cocktail III

EDTA-Free, liquid, for the inhibition of aspartic, cysteine, and serine proteases and aminopeptidases in mammalian cells and tissues.

• 別名: EDTA free Protease inhibitor, EDTA-Free Protease Inhibitor Mix, Protease inhibitor cocktail III
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• 製品名: Protease Inhibitor Cocktail Set III, EDTA-Free, Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.
• フォーム: liquid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Note: available as 1 x 1 ml or as a set of 5 x 1 ml.

This protease inhibitor cocktail set is a specially formulated cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cells and tissue extracts, but is also suitable for bacterial extracts. It is provided as a single vial or as a set of 5 vials. Each vial contains 1 ml protease inhibitor cocktail solubilized in DMSO with a small amount of water.

特異性

Inhibits a broad spectrum of serine proteases, cysteine proteases, and aspartic proteases, as well as aminopeptidases.

アプリケーション

Protease Inhibitor Cocktail Set III, EDTA-Free has been used:

• as a component in CelLytic MT cell lysis reagent or lysis buffer to homogenize kidney organoids for immunoblot analysis
• as a component in homogenization buffer to homogenize minced mouse quadriceps femoris muscle tissue
• in lysis buffer to extract proteins for immunoblotting

生物化学的／生理学的作用

Protease inhibitor cocktails and specialized protease inhibitors play a crucial role in various proteomic studies conducted by scientists. These studies encompass protein expression and characterization, biomarker discovery, identification of post-translational modifications, protein expression profiling, and quantitative protein measurement. The inclusion of a protease inhibitor cocktail or specific protease inhibitor guarantees the preservation of protein integrity, allowing for accurate downstream analysis and comprehensive sample characterization.

特徴および利点

• EDTA-Free: recommended for IMAC applications.
• Flexible: compatible with bacterial extracts, mammalian cell extracts, and tissue extracts.
• Fast: no waiting time for tablets to dissolve.
• Economical: ready-to-use format reduces wastage.
• Effective: one ml is sufficient for approximately 20g of tissue.
• Complete: contains six protease inhibitors to inhibit aspartic, cysteine, serine proteases, and aminopeptidases.

警告

Toxicity: Irritant (B)

再構成

Following initial thaw, aliquot and freeze (-20°C).

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H315,H319
• 注意書き: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• 危険有害性の分類: Eye Irrit. 2 - Skin Irrit. 2
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): 188.6 °F - (refers to pure substance)
• 引火点(℃): 87 °C - (refers to pure substance)