203390

(±)-Blebbistatin

≥97% (HPLC), solid, Myosin II inhibitor, Calbiochem^®

• 別名: (±)-Blebbistatin
• 実験式（ヒル表記法）: C₁₈H₁₆N₂O₂
• CAS番号: 674289-55-5
• 分子量: 292.33
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• 製品名: (±)-Blebbistatin, (±)-Blebbistatin, CAS 674289-55-5, is a cell-permeable, selective, and reversible inhibitor of nonmuscle myosin II. Blocks cell blebbing and disrupts cell migration & cytoKinesis in vertebrate cells.
• 品質水準: 100
• アッセイ: ≥97% (HPLC)
• フォーム: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: yellow
• 溶解性: methanol: 1.5 mg/mL; 100% DMSO: 100 mg/mL; 90% DMSO: 75 mg/mL
• 輸送温度: wet ice
• 保管温度: −20°C
• InChI: 1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3
• InChI Key: LZAXPYOBKSJSEX-UHFFFAOYSA-N

詳細

詳細

A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of non-muscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly.

A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of nonmuscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤ 100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly. A 50 mM (5 mg/342 µl) solution of (±)-Blebbistatin (Cat. No. 203389) in 90% DMSO is also available.

生物化学的／生理学的作用

Cell permeable: yes

Primary Target
ATPase

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: <100 µM inhibiting the ATPase and gliding motility of human platelets

包装

Packaged under inert gas

再構成

Following reconstitution in 90% DMSO, aliquot and freeze (-20°C). Stock solutions in 90% DMSO are stable for up to 1 month at -20°C. Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.

その他情報

Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl.12, 271a.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable